Spatial features for Escherichia coli genome organization
نویسندگان
چکیده
منابع مشابه
Multiscale spatial organization of RNA polymerase in Escherichia coli.
Nucleic acid synthesis is spatially organized in many organisms. In bacteria, however, the spatial distribution of transcription remains obscure, owing largely to the diffraction limit of conventional light microscopy (200-300 nm). Here, we use photoactivated localization microscopy to localize individual molecules of RNA polymerase (RNAP) in Escherichia coli with a spatial resolution of ∼40 nm...
متن کاملSpatial organization of transcription elongation complex in Escherichia coli.
During RNA synthesis in the ternary elongation complex, RNA polymerase enzyme holds nucleic acids in three contiguous sites: the double-stranded DNA-binding site (DBS) ahead of the transcription bubble, the RNA-DNA heteroduplex-binding site (HBS), and the RNA-binding site (RBS) upstream of HBS. Photochemical cross-linking allowed mapping of the DNA and RNA contacts to specific positions on the ...
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Escherichia albertii is a recently recognized close relative of Escherichia coli. This emerging enteropathogen possesses a type III secretion system (T3SS) encoded by the locus of enterocyte effacement, similar to enteropathogenic and enterohemorrhagic E. coli (EPEC and EHEC). Shiga toxin-producing strains have also been identified. The genomic features of E. albertii, particularly differences ...
متن کاملSpatial anisotropy in Escherichia coli.
There has been little indication of spatial orientation of structural elements within cells of Escherichia coli. Typical electron micrographs of E. coli indicate a randomly oriented nuclear material embedded in a substantially isotropic cytoplasm (3), although exceptions to this have been reported (2). Interpretation of recent microscopic observations of plasmolyzed cells of E. coli now lead us...
متن کاملMacrodomain organization of the Escherichia coli chromosome.
We have explored the Escherichia coli chromosome architecture by genetic dissection, using a site-specific recombination system that reveals the spatial proximity of distant DNA sites and records interactions. By analysing the percentages of recombination between pairs of sites scattered over the chromosome, we observed that DNA interactions were restricted to within subregions of the chromosom...
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ژورنال
عنوان ژورنال: BMC Genomics
سال: 2015
ISSN: 1471-2164
DOI: 10.1186/s12864-015-1258-1